Genome-scale CRISPRi screening: A powerful tool in engineering microbiology

Deciphering gene function is fundamental to engineering of microbiology. The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adapted for repression across a range hosts, creating versatile tool called CRISPR interference (CRISPRi) that enables genome-scale analysis function. This approach yielded significant advances in the design CRISPRi libraries, as well applications screening medical and industrial review provides an overview recent progress made pooled arrayed microorganisms highlights representative studies have employed this method. Additionally, challenges associated with are discussed, potential solutions optimizing strategy proposed.